RPL35A is a key promotor involved in the development and progression of gastric cancer.

ABSTRACT:

Background

RPL35A has been reported to work as a biomarker in tumor angiogenesis. However, little work has been performed on the expression level and functional importance of RPL35A in gastric cancer (GC).

Methods

The protein expression level of RPL35A was detected by immunohistochemical staining and western blot analysis. The Celigo cell counting assay was used to assess cell proliferation. Both the wound healing assay and the transwell assay were conducted to evaluate cell migration. Flow cytometric analysis was utilized to detect cell apoptosis and cell cycle. A mouse xenograft model was constructed for in vivo experiments.

Results

The results demonstrated that RPL35A expression was abundantly up-regulated in GC and positively related to tumor infiltrate. In additi

SUBMITTER: Wu F 

PROVIDER: S-EPMC8447681 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature