Multiscale model of defective interfering particle replication for influenza A virus infection in animal cell culture.

ABSTRACT: Cell culture-derived defective interfering particles (DIPs) are considered for antiviral therapy due to their ability to inhibit influenza A virus (IAV) production. DIPs contain a large internal deletion in one of their eight viral RNAs (vRNAs) rendering them replication-incompetent. However, they can propagate alongside their homologous standard virus (STV) during infection in a competition for cellular and viral resources. So far, experimental and modeling studies for IAV have focused on either the intracellular or the cell population level when investigating the interaction of STVs and DIPs. To examine these levels simultaneously, we conducted a series of experiments using highly different multiplicities of infections for STVs and DIPs to characterize virus replication in Madin-Darby Ca

SUBMITTER: Rudiger D 

PROVIDER: S-EPMC8448327 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature