Novel reassortant of H9N2 avian influenza viruses isolated from chickens and quails in Egypt.

ABSTRACT:

Background and aim

Poultry infections with H9N2 avian influenza viruses (AIVs) are endemic in Egypt. This study determined the genetic changes in the sequences of H9N2 AIVs isolated from chicken and quails in Egypt, including determining genetic reassortment and detecting the main genetic changes in hemagglutinin (HA) and neuraminidase (NA) genes.

Materials and methods

Swab samples were collected from chicken and quails, examined through reverse transcription-polymerase chain reaction, and AIVs from positive samples were isolated in embryonated chicken eggs. Complete genome sequencing and phylogenetic analyses were conducted for two H9N2 AIV isolates, and sequences of HA and NA gene segments were analyzed in another two isolates.

Results

A novel reassortant virus was

SUBMITTER: Elsayed M 

PROVIDER: S-EPMC8448633 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature