Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats.

ABSTRACT:

Aim

The study investigated the genetic polymorphism of the kisspeptin (KiSS1) gene and its relationship with litter size in Cyprus and Iraqi black goats.

Materials and methods

Blood samples (n=124) were collected from the two goat breeds reared at the Agricultural Research-Ruminant Research Station Breeding Station, Baghdad, Iraq. Genomic DNA was isolated using a DNA extraction kit. Polymerase chain reaction (PCR) was used to amplify the KiSS1 gene. All PCR products were sequenced and samples were used for further analysis using NCBI-Blast online on the exon 1 (595 bp) region of the KiSS1 gene.

Results

The results of this study revealed a significantly (P<0.05) larger litter size of the Cyprus goat breed than in the Iraqi black goats in the

SUBMITTER: Rahawy MA 

PROVIDER: S-EPMC8448648 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature