Argininosuccinate lyase is a metabolic vulnerability in breast development and cancer.

ABSTRACT: Epithelial-to-mesenchymal transition (EMT) is fundamental to both normal tissue development and cancer progression. We hypothesized that EMT plasticity defines a range of metabolic phenotypes and that individual breast epithelial metabolic phenotypes are likely to fall within this phenotypic landscape. To determine EMT metabolic phenotypes, the metabolism of EMT was described within genome-scale metabolic models (GSMMs) using either transcriptomic or proteomic data from the breast epithelial EMT cell culture model D492. The ability of the different data types to describe breast epithelial metabolism was assessed using constraint-based modeling which was subsequently verified using ¹³C isotope tracer analysis. The application of proteomic data to GSMMs provided relatively higher

SUBMITTER: Karvelsson ST 

PROVIDER: S-EPMC8448827 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature