Protein functional dynamics from the rigorous global analysis of DEER data: Conditions, components, and conformations.

ABSTRACT: The potential of spin labeling to reveal the dynamic dimension of macromolecules has been recognized since the dawn of the methodology in the 1960s. However, it was the development of pulsed electron paramagnetic resonance spectroscopy to detect dipolar coupling between spin labels and the availability of turnkey instrumentation in the 21st century that realized the full promise of spin labeling. Double electron-electron resonance (DEER) spectroscopy has seen widespread applications to channels, transporters, and receptors. In these studies, distance distributions between pairs of spin labels obtained under different biochemical conditions report the conformational states of macromolecules, illuminating the key movements underlying biological function. These experimental studies have spurr

SUBMITTER: Hustedt EJ 

PROVIDER: S-EPMC8449309 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature