Unknown

Dataset Information

0

The vitellogenin receptor functionality of the migratory locust depends on its phosphorylation by juvenile hormone.


ABSTRACT: Vitellogenin receptor (VgR) plays a pivotal role in ovarian vitellogenin (Vg) uptake and vertical transmission of pathogenic microbes and Wolbachia symbionts. However, the regulatory mechanisms of VgR action as an endocytic receptor and translocation from oocyte cytoplasm to the membrane remain poorly understood. Here, by using the migratory locust Locusta migratoria as a model system, we report that juvenile hormone (JH) promotes VgR phosphorylation at Ser1361 in the second EGF-precursor homology domain. A signaling cascade including GPCR, PLC, extracellular calcium, and PKC-ι is involved in JH-stimulated VgR phosphorylation. This posttranslational regulation is a prerequisite for VgR binding to Vg on the external surface of the oocyte membrane and subsequent VgR/Vg endocytosis. Acidification, a condition in endosomes, induces VgR dephosphorylation along with the dissociation of Vg from VgR. Phosphorylation modification is also required for VgR recycling from oocyte cytoplasm to the membrane. Additionally, VgR phosphorylation and its requirement for Vg uptake and VgR recycling are evolutionarily conserved in other representative insects including the cockroach Periplaneta americana and the cotton bollworm Helicoverpa armigera This study fills an important knowledge gap of low-density lipoprotein receptors in posttranslational regulation, endocytosis, and intracellular recycling.

SUBMITTER: Jing YP 

PROVIDER: S-EPMC8449341 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

The vitellogenin receptor functionality of the migratory locust depends on its phosphorylation by juvenile hormone.

Jing Yu-Pu YP   Wen Xinpeng X   Li Lunjie L   Zhang Shanjing S   Zhang Ci C   Zhou Shutang S  

Proceedings of the National Academy of Sciences of the United States of America 20210901 37


Vitellogenin receptor (VgR) plays a pivotal role in ovarian vitellogenin (Vg) uptake and vertical transmission of pathogenic microbes and <i>Wolbachia</i> symbionts. However, the regulatory mechanisms of VgR action as an endocytic receptor and translocation from oocyte cytoplasm to the membrane remain poorly understood. Here, by using the migratory locust <i>Locusta migratoria</i> as a model system, we report that juvenile hormone (JH) promotes VgR phosphorylation at Ser<sup>1361</sup> in the se  ...[more]

Similar Datasets

| S-EPMC4207617 | biostudies-literature
| S-EPMC6658565 | biostudies-literature
| S-EPMC1852330 | biostudies-literature
| S-EPMC3704040 | biostudies-literature
| S-EPMC4813133 | biostudies-literature
| S-EPMC8683598 | biostudies-literature
| S-EPMC4007805 | biostudies-literature
| S-EPMC3248701 | biostudies-literature
| S-EPMC11203946 | biostudies-literature
| S-EPMC5024112 | biostudies-literature