Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering.

ABSTRACT: Pentameric ligand-gated ion channels undergo subtle conformational cycling to control electrochemical signal transduction in many kingdoms of life. Several crystal structures have now been reported in this family, but the functional relevance of such models remains unclear. Here, we used small-angle neutron scattering (SANS) to probe ambient solution-phase properties of the pH-gated bacterial ion channel GLIC under resting and activating conditions. Data collection was optimized by inline paused-flow size-exclusion chromatography, and exchanging into deuterated detergent to hide the micelle contribution. Resting-state GLIC was the best-fit crystal structure to SANS curves, with no evidence for divergent mechanisms. Moreover, enhanced-sampling molecular-dynamics simulations enabled differen

SUBMITTER: Lycksell M 

PROVIDER: S-EPMC8449418 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature