LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis.

ABSTRACT:

Background

Long noncoding RNAs (lncRNAs) are related to colorectal cancer (CRC) development. However, the role and mechanism of lncRNA LINC01224 in CRC development are largely unknown.

Methods

LINC01224, Yin Yang 1 (YY1), microRNA (miR)-485-5p, and myosins of class VI (MYO6) levels were examined using quantitative reverse transcription polymerase chain reaction and western blotting. Functional analyses were processed through CCK-8, colony formation, flow cytometry, transwell, and xenograft analyses. Dual-luciferase reporter, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation, and pull-down assays were conducted to analyze the binding interaction.

Results

LINC01224 abundance was elevated in CRC tissue samples and cell lines. Elevated LINC01224 might indicate

SUBMITTER: Gu J 

PROVIDER: S-EPMC8449439 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature