LncRNA LBX2-AS1 promotes colorectal cancer progression and 5-fluorouracil resistance.

ABSTRACT:

Background

Recent reports suggest that the long non-coding RNA LBX2 antisense RNA 1 (LBX2-AS1) acts as an important regulator in cancer progression, but its significance in colorectal cancer (CRC) remains undetermined.

Methods

LBX2-AS1 expression levels in CRC were determined from the GEPIA database and CRC tissues to investigate clinical relevance. meRIP-PCR assays investigated the molecular mechanisms underlying the function of m6A in LBX2-AS1. Loss of function experiments was used to define the role of LBX2-AS1 in the progression of CRC. The ceRNA function of LBX2-AS1 was evaluated by RNA immunoprecipitation. In vitro and PDX models were used to determine if LBX2-AS1 promotes 5-fluorouracil resistance.

Results

Data from the TCGA and our institutional patient cohort

SUBMITTER: Ma YN 

PROVIDER: S-EPMC8449476 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature