Codon usage and protein length-dependent feedback from translation elongation regulates translation initiation and elongation speed.

ABSTRACT: Essential cellular functions require efficient production of many large proteins but synthesis of large proteins encounters many obstacles in cells. Translational control is mostly known to be regulated at the initiation step. Whether translation elongation process can feedback to regulate initiation efficiency is unclear. Codon usage bias, a universal feature of all genomes, plays an important role in determining gene expression levels. Here, we discovered that there is a conserved but codon usage-dependent genome-wide negative correlation between protein abundance and CDS length. The codon usage effects on protein expression and ribosome flux on mRNAs are influenced by CDS length; optimal codon usage preferentially promotes production of large proteins. Translation of mRNAs with long CDS

SUBMITTER: Lyu X 

PROVIDER: S-EPMC8450115 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature