Fine-tuning of dual-SMAD inhibition to differentiate human pluripotent stem cells into neural crest stem cells.

ABSTRACT:

Objectives

The derivation of neural crest stem cells (NCSCs) from human pluripotent stem cells (hPSCs) has been commonly induced by WNT activation in combination with dual-SMAD inhibition. In this study, by fine-tuning BMP signalling in the conventional dual-SMAD inhibition, we sought to generate large numbers of NCSCs without WNT activation.

Materials and methods

In the absence of WNT activation, we modulated the level of BMP signalling in the dual-SMAD inhibition system to identify conditions that efficiently drove the differentiation of hPSCs into NCSCs. We isolated two NCSC populations separately and characterized them in terms of global gene expression profiles and differentiation ability.

Results

Our modified dual-SMAD inhibition containing a lower dose of BMP i

SUBMITTER: Kim HM 

PROVIDER: S-EPMC8450125 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature