Amphiregulin promotes hair regeneration of skin-derived precursors via the PI3K and MAPK pathways.

ABSTRACT:

Objectives

There are significant clinical challenges associated with alopecia treatment, including poor efficiency of related drugs and insufficient hair follicles (HFs) for transplantation. Skin-derived precursors (SKPs) exhibit great potential as stem cell-based therapies for hair regeneration; however, the proliferation and hair-inducing capacity of SKPs gradually decrease during culturing.

Materials and methods

We describe a 3D co-culture system accompanied by kyoto encyclopaedia of genes and genomes and gene ontology enrichment analyses to determine the key factors and pathways that enhance SKP stemness and verified using alkaline phosphatase assays, Ki-67 staining, HF reconstitution, Western blot and immunofluorescence staining. The upregulated genes were confirmed uti

SUBMITTER: Lu Q 

PROVIDER: S-EPMC8450126 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature