Development of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of <i>Phytopythium vexans</i>.

ABSTRACT: Brown root rot caused by Phytopythium vexans is a new destructive root disease on many plants such as Gingko, Citrus, kiwifruit, and ramie. The establishment of loop-mediated isothermal amplification (LAMP) technology for detecting P. vexans can help monitor and control brown root rot quickly, efficiently, and accurately. LAMP technology is known for its simplicity, sensitivity, and speed; and it does not require any specialized equipment - a water bath or a thermoblock is sufficient for isothermal amplifications. LAMP products can be visualized by using hydroxy naphthol blue (HNB) dye or agarose gel electrophoresis. In this study, by searching and comparing the internal transcribed spacer (ITS) sequences of P. vexans and the related species in oomycete genera Pythi

SUBMITTER: Wang T 

PROVIDER: S-EPMC8450588 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature