Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer.

ABSTRACT:

Objective

The objective was to explore the expression and potential functions of long noncoding RNA (lncRNA) and mRNAs in human breast cancer (BC).

Methods

Differentially expressed lncRNAs and mRNAs were identified and annotated in BC tissues by using the Agilent human lncRNA assay (Agilent Technologies, Santa Clara, CA, USA) and RNA sequencing. After identification of lncRNAs and mRNAs through quantitative reverse transcription polymerase chain reaction, we conducted a series of functional experiments to confirm the effects of knockdown of one lncRNA, TCONS_00029809, on the progression of BC.

Results

We discovered 238 lncRNAs and 200 mRNAs that were differentially expressed in BC tissues and para-carcinoma tissue. We showed that differentially expressed mRNAs were re

SUBMITTER: Guo J 

PROVIDER: S-EPMC8451004 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature