TAGLN mediated stiffness-regulated ovarian cancer progression via RhoA/ROCK pathway.

ABSTRACT:

Background

Ovarian cancer (OC) progression is an unmet medical challenge. Since omental metastases were palpated harder than their primary counterparts during cytoreductive surgery of patients with epithelial ovarian cancer (EOC), we were inspired to investigate OC progression from the perspective of biomechanics.

Methods

Atomic Force Microscope (AFM) was used to measure the Young's modulus of tissues. The collagen-coated polyacrylamide hydrogel (PA gel) system was prepared to mimic the soft and stiff substrates in vitro. The effect of TAGLN was evaluated both in vitro and in vivo using transwell assay, immunofluorescence, western blot analysis and immunohistochemistry.

Results

We quantitatively confirmed that omental metastases were stiffer and more abundant in desmo

SUBMITTER: Wei X 

PROVIDER: S-EPMC8451140 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature