Laser Emission of Thioflavin T Uncovers Protein Aggregation in Amyloid Nucleation Phase.

ABSTRACT: There is currently no definitive test for early detection of neurodegeneration which is linked with protein aggregation. Finding methods capable of detecting intermediate states of protein aggregates, named oligomers, is critical for the early stage diagnosis of over 30 neurodegenerative diseases including Alzheimer's or Parkinson's. Currently, fluorescence-based imaging using Thioflavin T (ThT) dye is the gold standard for detecting protein aggregation. It is used to detect aggregation in vitro and in various tissues, including the cerebrospinal fluid (CSF), whereby the disease-related protein recombinant is seeded with the patient's fluid. The major drawback of ThT is its lack of sensitivity to oligomeric forms of protein aggregates. Here, we overcome this limitation by transferring a Th

SUBMITTER: Hanczyc P 

PROVIDER: S-EPMC8451393 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature