Molecular Epidemiology of Human Sapovirus among Children with Acute Gastroenteritis in Western Canada.

ABSTRACT: Sapovirus is increasingly recognized as an important cause of acute gastroenteritis (AGE) worldwide; however, studies of sapovirus prevalence, genetic diversity, and strain-specific clinical implications have been scarce. To fill this knowledge gap, we used reverse transcription-real-time PCR and sequencing of the partial major capsid protein VP1 gene to analyze stool specimens and rectal swabs obtained from 3,347 children with AGE and 1,355 asymptomatic controls (all <18 years old) collected between December 2014 and August 2018 in Alberta, Canada. Sapovirus was identified in 9.5% (317/3347) of the children with AGE and 2.9% of controls. GI.1 (36%) was the predominant genotype identified, followed by GI.2 (18%), GII.5 (8%), and GII.3 (6%). Rare genotypes GII.1, GII.2, GV.1, GII.4, GIV.1,

SUBMITTER: Zhuo R 

PROVIDER: S-EPMC8451440 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature