Hsp70 modulates immune response in pancreatic cancer through dendritic cells.

ABSTRACT: Heat shock protein 70 (Hsp70), a protein chaperone, is known to promote cell survival and tumor progression. However, its role in the tumor microenvironment (TME) is largely unknown. We specifically evaluated Hsp70 in the TME by implanting tumors in wild-type (WT) controls or Hsp70^-/- animals, thus creating a TME with or without Hsp70. Loss of Hsp70 led to significantly smaller tumors; there were no differences in stromal markers, but interestingly, depletion of CD8 + T-cells abrogated this tumor suppressive effect, indicating that loss of Hsp70 in the TME affects tumor growth through the immune cells. Compared to WT, adoptive transfer of Hsp70^-/- splenocytes exhibited greater antitumor activity in immunodeficient NSG and Rag 1^-/- mice. Hsp70^-/-

SUBMITTER: Giri B 

PROVIDER: S-EPMC8451449 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature