AmpliconDesign - an interactive web server for the design of high-throughput targeted DNA methylation assays.

ABSTRACT: Targeted analysis of DNA methylation patterns based on bisulfite-treated genomic DNA (BT-DNA) is considered as a gold-standard for epigenetic biomarker development. Existing software tools facilitate primer design, primer quality control or visualization of primer localization. However, high-throughput design of primers for BT-DNA amplification is hampered by limits in throughput and functionality of existing tools, requiring users to repeatedly perform specific tasks manually. Consequently, the design of PCR primers for BT-DNA remains a tedious and time-consuming process. To bridge this gap, we developed AmpliconDesign, a webserver providing a scalable and user-friendly platform for the design and analysis of targeted DNA methylation studies based on BT-DNA, e.g. deep amplic

SUBMITTER: Schonung M 

PROVIDER: S-EPMC8451469 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature