Dbx2 regulation in limbs suggests interTAD sharing of enhancers.

ABSTRACT:

Background

During tetrapod limb development, the HOXA13 and HOXD13 transcription factors are critical for the emergence and organization of the autopod, the most distal aspect where digits will develop. Since previous work had suggested that the Dbx2 gene is a target of these factors, we set up to analyze in detail this potential regulatory interaction.

Results

We show that HOX13 proteins bind to mammalian-specific sequences at the vicinity of the Dbx2 locus that have enhancer activity in developing digits. However, the functional inactivation of the DBX2 protein did not elicit any particular phenotype related to Hox genes inactivation in digits, suggesting either redundant or compensatory mechanisms. We report that the neighboring Nell2 and Ano6 genes are also expressed in

SUBMITTER: Beccari L 

PROVIDER: S-EPMC8451760 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature