Increased Th22 cell numbers in a general pediatric population with filaggrin haploinsufficiency: The Generation R Study.

ABSTRACT:

Background

Mutations in the filaggrin gene (FLG) affect epidermal barrier function and increase the risk of atopic dermatitis (AD). We hypothesized that FLG mutations affect immune cell composition in a general pediatric population. Therefore, we investigated whether school-aged children with and without FLG mutations have differences in T- and B-cell subsets.

Methods

This study was embedded in a population-based prospective cohort study, the Generation R Study, and included 523 children of European genetic ancestry aged 10 years. The most common FLG mutations in the European population (R501X, S1085CfsX36, R2447X, and S3247X) were genotyped. Additionally, 11-color flow cytometry was performed on peripheral blood samples to determine helper T (Th), regulatory T (Treg), and C

SUBMITTER: Looman KIM 

PROVIDER: S-EPMC8451856 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature