Enrichment of circulating trophoblasts from maternal blood using laminar microscale vortices.

ABSTRACT:

Objective

Enrichment of circulating trophoblasts (CTs) from maternal blood at week 11-13 of gestation, using laminar microscale vortices, and evaluation of the performance of the VTX-1 Liquid Biopsy System in terms of CT recovery and purity.

Method

Eight mililiter of blood was collected from 15 pregnant women and processed with the VTX-1 Liquid Biopsy System. Y-chromosome specific quantitative PCR was performed to estimate the number of enriched male CTs. To evaluate the VTX-1 performance, the target cell recovery was characterized by spiking experiments with a trophoblast cell line. Furthermore, the total quantity of DNA after enrichment was used to calculate the number of retained maternal cells.

Results

Successful recovery of male CTs was established in 7 out of 10

SUBMITTER: Vander Plaetsen AS 

PROVIDER: S-EPMC8451878 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature