Generation of a GLO-2 deficient mouse reveals its effects on liver carbonyl and glutathione levels.

ABSTRACT:

Objective

Hydroxyacylglutathione hydrolase (aka as GLO-2) is a component of the glyoxalase pathway involved in the detoxification of the reactive oxoaldehydes, glyoxal and methylglyoxal. These reactive metabolites have been linked to a variety of pathological conditions, including diabetes, cancer and heart disease and may be involved in the aging process. The objective of this study was to generate a mouse model deficient in GLO-2 to provide insight into the function of GLO-2 and to determine if it is potentially linked to endogenous oxalate synthesis which could influence urinary oxalate excretion.

Methods

A GLO-2 knock out mouse was generated using CRISPR/Cas 9 techniques. Tissue and 24-h urine samples were collected under baseline conditions from adult male and female an

SUBMITTER: Li X 

PROVIDER: S-EPMC8453187 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature