Decreased Jagged1 expression in vascular smooth muscle cells delays endothelial regeneration in arteriovenous graft.

ABSTRACT:

Aims

It is well-established that endothelial dysfunction promotes activation of vascular smooth muscle cell (VSMC). Whether decreased accumulation of VSMCs affects endothelial regeneration and functions in arteriovenous graft (AVG) remodelling has not been studied. We sought to identify mechanisms by which the Notch ligand, Jagged1, in VSMCs regulates endothelial cell (EC) functions in AVGs.

Methods and results

AVGs were created in transgenic mice bearing VSMC-specific knockout (KO) or overexpression of Jagged1. VSMC migration, EC regeneration, and its barrier functions as well as AVG remodelling were evaluated. Jagged1 expression was induced in VSMCs of neointima in the AVGs. Jagged1 KO in VSMCs inhibited the accumulation of extracellular matrix as well as VSMC migration. F

SUBMITTER: Guo Q 

PROVIDER: S-EPMC8453291 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature