Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone.

ABSTRACT:

Background and purpose

l-cysteine or hydrogen sulfide (H₂ S) donors induce a biphasic effect on precontracted isolated vessels. The contractile effect occurs within a concentration range of 10 nM to 3 μM followed by vasodilatation at 30-100 μM. Here, we have investigated the signalling involved in the H₂ S-induced contraction.

Experimental approach

Vascular response to NaHS or l-cysteine is evaluated on isolated precontracted with phenylephrine vessel rings harvested from wild type, cystathionine γ-lyase (CSE^-/- ), soluble guanylyl cyclase (sGC_α1^-/- ) and endothelial nitric oxide synthase (eNOS^-/- ) knock-out mice. The cAMP, cGMP and inosine 3',5'-cyclic monophosphate (cIMP) levels are simultaneously quantifie

SUBMITTER: Mitidieri E 

PROVIDER: S-EPMC8453910 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature