The plasma exosomal miR-1180-3p serves as a novel potential diagnostic marker for cutaneous melanoma.

ABSTRACT:

Background

Exosomes are a promising tool in disease detection because they are noninvasive, cost-effective, sensitive and stable in body fluids. MicroRNAs (miRNAs) are the main exosomal component and participate in tumor development. However, the exosomal miRNA profile among Asian melanoma patients remains unclear.

Methods

Exosomal miRNAs from the plasma of melanoma patients (n = 20) and healthy individuals (n = 20) were isolated and subjected to small RNA sequencing. Real-time PCR was performed to identify the differential miRNAs and to determine the diagnostic efficiency. Proliferation, scratch and Transwell assays were performed to detect the biological behavior of melanoma cells.

Results

Exosomal miRNA profiling revealed decreased miR-1180-3p expression as a poten

SUBMITTER: Guo Y 

PROVIDER: S-EPMC8454000 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature