Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays.

ABSTRACT:

Background

The pathways that control protein transport across the blood-brain barrier (BBB) remain poorly characterized. Despite great advances in recapitulating the human BBB in vitro, current models are not suitable for systematic analysis of the molecular mechanisms of antibody transport. The gaps in our mechanistic understanding of antibody transcytosis hinder new therapeutic delivery strategy development.

Methods

We applied a novel bioengineering approach to generate human BBB organoids by the self-assembly of astrocytes, pericytes and brain endothelial cells with unprecedented throughput and reproducibility using micro patterned hydrogels. We designed a semi-automated and scalable imaging assay to measure receptor-mediated transcytosis of antibodies. Finally, we develo

SUBMITTER: Simonneau C 

PROVIDER: S-EPMC8454074 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature