In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography.

ABSTRACT: The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs in 13 diderm bacterial species and classified several major ultrastructures: (1) tubes with a uniform diameter (with or without an internal scaff

SUBMITTER: Kaplan M 

PROVIDER: S-EPMC8455137 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature