Progressive Cellular Senescence Mediates Renal Dysfunction in Ischemic Nephropathy.

ABSTRACT:

Background

Peripheral vascular diseases may induce chronic ischemia and cellular injury distal to the arterial obstruction. Cellular senescence involves proliferation arrest in response to stress, which can damage neighboring cells. Renal artery stenosis (RAS) induces stenotic-kidney dysfunction and injury, but whether these arise from cellular senescenceand their temporal pattern remain unknown.

Methods

Chronic renal ischemia was induced in transgenic INK-ATTAC and wild type C57BL/6 mice by unilateral RAS, and kidney function (in vivo micro-MRI) and tissue damage were assessed. Mouse healthy and stenotic kidneys were analyzed using unbiased single-cell RNA-sequencing. To demonstrate translational relevance, cellular senescence was studied in human stenotic kidneys.

SUBMITTER: Kim SR 

PROVIDER: S-EPMC8455278 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature