Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells.

ABSTRACT: The molecular assembly of cells depends not only on the balance between anabolism and catabolism but to a large degree on the building blocks available in the environment. For cultured mammalian cells, this is largely determined by the composition of the applied growth medium. Here, we study the impact of lipids in the medium on mitochondrial membrane architecture and function by combining LC-MS/MS lipidomics and functional tests with lipid supplementation experiments in an otherwise serum-free and lipid-free cell culture model. We demonstrate that the composition of mitochondrial cardiolipins strongly depends on the lipid environment in cultured cells and favors the incorporation of essential linoleic acid over other fatty acids. Simultaneously, the mitochondrial respiratory complex I act

SUBMITTER: Oemer G 

PROVIDER: S-EPMC8455370 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature