Secretory helpers for enhanced production of heterologous proteins in Yarrowia lipolytica.

ABSTRACT: Depending on the suboptimal factor, the target protein secretion can be over 1000-fold below the theoretical maximum. The bottlenecks may be alleviated by co-overexpression of "secretory helpers" (SHs). Here we proposed twelve SHs, functionally spanning the whole transcription-translation-translocation-folding-maturation-excretion pipeline. The genes were co-transformed with an easy-to-track reporter, and tested less than two temperatures. Our results indicated a clear distinction in the effects triggered by SHs involved in either synthesis or trafficking of the heterologous polypeptides. For superior operation of synthesis-related SHs, namely RPL3, SSA5 and SSA8, the secretory pathway's capacity must be released by applying decreased temperature (

SUBMITTER: Korpys-Wozniak P 

PROVIDER: S-EPMC8455373 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature