Micro flow photochemical synthesis of Ca-sensitive fluorescent sensor particles.

ABSTRACT: Fluorescence probes have widely been used for detecting and imaging Ca²⁺-enriched parts of cells but more rarely for quantitative determination of concentrations. In this study we show how this can be achieved by a novel approach using hydrogel particles. In a microfluidic co-flow arrangement spherical droplets were generated from an aqueous solution of acrylamide, N,N'-methylenebisacrylamide crosslinker and photoinitiator and subsequently photo-cured in situ yielding gel particles in a sub millimeter range. These particles were separated, dried under reduced pressure and re-swollen in water containing Rhod-5N tri potassium salt as calcium ion selective fluorescence probe. After that the particles were dried again and stored for further investigations. Upon exposure of dr

SUBMITTER: Kronfeld KP 

PROVIDER: S-EPMC8456324 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature