The effects of removing dead bacteria by propidium monoazide on the profile of salivary microbiome.

ABSTRACT:

Background

Oral microbiome played an important role in maintaining healthy state and might exhibit certain changes under circumstances of diseases. However, current microbiological research using sequencing techniques did not regard dead bacteria as a separate part, causing findings based on subsequent analyses on dynamic equilibrium and functional pathways of microbes somewhat questionable. Since treatment by propidium monoazide (PMA) was able to remove dead bacteria effectively, it would be worth studying how the sequencing results after PMA treatment differed from those focusing on the whole microbiota.

Methods

Unstimulated whole saliva samples were obtained from 18 healthy people from 3 age groups (children, adults, and the elderly). After removal of dead bacteria by pro

SUBMITTER: Ren Q 

PROVIDER: S-EPMC8456568 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature