Characterization of the chicken T cell receptor γ repertoire by high-throughput sequencing.

ABSTRACT:

Background

As one of "γδ-high" species, chicken is an excellent model for the study of γδ T cells in non-mammalian animals. However, a comprehensive characterization of the TCRγδ repertoire is still missing in chicken. The objective of this study was to characterize the expressed TCRγ repertoire in chicken thymus using high-throughput sequencing.

Methods

In this study, we first obtained the detailed genomic organization of the TCRγ locus of chicken based on the latest assembly of the red jungle fowl genome sequences (GRCg6a) and then characterized the TCRγ repertoire in the thymus of four chickens by using 5' Rapid Amplification of cDNA Ends (5' RACE) along with high-throughput sequencing (HTS).

Results

The chicken TCRγ locus contains a single Cγ gene, three functiona

SUBMITTER: Zhang T 

PROVIDER: S-EPMC8456604 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature