Intrinsically Disordered Osteopontin Fragment Orders During Interfacial Calcium Oxalate Mineralization.

ABSTRACT: Calcium oxalate (CaC₂ O₄ ) is the major component of kidney stone. The acidic osteopontin (OPN) protein in human urine can effectively inhibit the growth of CaC₂ O₄ crystals, thereby acting as a potent stone preventer. Previous studies in bulk solution all attest to the importance of binding and recognition of OPN at the CaC₂ O₄ mineral surface, yet molecular level insights into the active interface during CaC₂ O₄ mineralization are still lacking. Here, we probe the structure of the central OPN fragment and its interaction with Ca²⁺ and CaC₂ O₄ at the water-air interface using surface-specific non-linear vibrational spectroscopy. While OPN peptides remain largely disorde

SUBMITTER: Lu H 

PROVIDER: S-EPMC8457088 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature