Dual Valorization of Lignin as a Versatile and Renewable Matrix for Enzyme Immobilization and (Flow) Bioprocess Engineering.

ABSTRACT: Lignin has emerged as an attractive alternative in the search for more eco-friendly and less costly materials for enzyme immobilization. In this work, the terephthalic aldehyde-stabilization of lignin is carried out during its extraction to develop a series of functionalized lignins with a range of reactive groups (epoxy, amine, aldehyde, metal chelates). This expands the immobilization to a pool of enzymes (carboxylase, dehydrogenase, transaminase) by different binding chemistries, affording immobilization yields of 64-100 %. As a proof of concept, a ω-transaminase reversibly immobilized on polyethyleneimine-lignin is integrated in a packed-bed reactor. The stability of the immobilized biocatalyst is tested in continuous-flow deamination reactions and maintains the same conversion for 100

SUBMITTER: Benitez-Mateos AI 

PROVIDER: S-EPMC8457236 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature