Identification of contaminating fungal DNA sequences in Zymolyase.

ABSTRACT: When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA.

SUBMITTER: Rimek D 

PROVIDER: S-EPMC84574 | biostudies-literature | 1999 Mar

REPOSITORIES: biostudies-literature