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Molecular cloning and characterization of common marmoset SULT1C subfamily members that catalyze the sulfation of thyroid hormones.

ABSTRACT: Cytosolic sulfotransferase SULT1C subfamily is one of the most flexible gene subfamilies during mammalian evolution. The physiological functions of SULT1C enzymes still remain to be fully understood. In this study, common marmoset (Callithrix jacchus), a promising primate animal model, was used to investigate the functional relevance of the SULT1C subfamily. Gene database search revealed 3 intact SULT1C genes and a pseudogene in its genome. These 4 genes were named SULT1C1, SULT1C2, SULT1C3P, and SULT1C5, according to the sequence homology and gene location. Since SULT1C5 is the orthologous gene for human SULT1C2P, we propose, here, to revisit the designation of human SULT1C2P to SULT1C5P. Purified recombinant SULT1C enzymes showed sulfating activities toward a variety of xenobiotic compounds and thyroid hormones. Kinetic analysis revealed high catalytic activities of SULT1C1 and SULT1C5 for 3,3'-T2. It appears therefore that SULT1C isoforms may play a role in the thyroid hormone metabolism in common marmoset.

SUBMITTER: Kurogi K

PROVIDER: S-EPMC8458394 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Molecular cloning and characterization of common marmoset SULT1C subfamily members that catalyze the sulfation of thyroid hormones.

Kurogi Katsuhisa K Manabe Yoko Y Liu Ming-Cheh MC Suiko Masahito M Sakakibara Yoichi Y

Bioscience, biotechnology, and biochemistry 20210901 10

Cytosolic sulfotransferase SULT1C subfamily is one of the most flexible gene subfamilies during mammalian evolution. The physiological functions of SULT1C enzymes still remain to be fully understood. In this study, common marmoset (Callithrix jacchus), a promising primate animal model, was used to investigate the functional relevance of the SULT1C subfamily. Gene database search revealed 3 intact SULT1C genes and a pseudogene in its genome. These 4 genes were named SULT1C1, SULT1C2, SULT1C3P, an ...[more]

PMID: 34370005

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Project description:IntroductionThyroid disease is an important condition to understand in elasmobranchs, with goiters being predominant. To identify dysfunction, measuring serum thyroid hormone levels is a standard of practice for diagnosing disease in most species. Although these levels have been reported in elasmobranch literature, the testing methodology is varied and values are not clinically useful for most aquarium species. In a group of aquarium-housed elasmobranchs, thyroid hormone levels had been persistently low or not detectable in otherwise healthy animals as well as animals with thyroid disease. The concern for reliability of these results to diagnose thyroid disease, prompted a shift to serum iodine levels as a proxy to determine thyroid health.MethodsThis study assesses thyroid hormone and iodine levels as compared to thyroid disease stage in elasmobranchs with and without dietary supplementation, to determine the efficacy of using these serum values to guide clinical decisions.ResultsSerum thyroid hormone results were lower than the readable range of the standard curve in both sharks and rays; thus reported values are usually extrapolated. Including additional standards down to the limit of sensitivity improved detection, however increasing the sample volume tested was determined to be the most important factor for obtaining measurable results in low-value thyroid hormone samples. Serum iodine levels are reported in three groups of southern stingrays (Hypanus americanus). Other elasmobranch species maintained in aquaria with and without thyroid disease were used for biological comparisons. Non-goiter, diseased animals reliably had elevated levels (over baseline) of thyroid hormones and iodine; in goiter cases, hormones were not useful. Additionally, it was found that cobalt levels were also elevated in some disease states and correlated positively with serum iodine levels.ConclusionCurrent available thyroid testing may not provide clinically useful values unless methodology is adjusted, or disease is severe. Serum iodine may be a useful marker to investigate thyroid health. Further, while thyroid disease may be identifiable with thyroid hormones, it is not straightforward or substantial enough alone for diagnosis.

Dataset Information

Molecular cloning and characterization of common marmoset SULT1C subfamily members that catalyze the sulfation of thyroid hormones.

Publications

Molecular cloning and characterization of common marmoset SULT1C subfamily members that catalyze the sulfation of thyroid hormones.

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