Site-specific transgenesis of the Drosophila melanogaster Y-chromosome using CRISPR/Cas9.

ABSTRACT: Despite the importance of Y-chromosomes in evolution and sex determination, their heterochromatic, repeat-rich nature makes them difficult to sequence (due, in part, to ambiguities in sequence alignment and assembly) and to genetically manipulate. Therefore, they generally remain poorly understood. For example, the Drosophila melanogaster Y-chromosome, one of the most extensively studied Y-chromosomes, is widely heterochromatic and composed mainly of highly repetitive sequences, with only a handful of expressed genes scattered throughout its length. Efforts to insert transgenes on this chromosome have thus far relied on either random insertion of transposons (sometimes harbouring 'landing sites' for subsequent integrations) with limited success or on chromosomal translocations, thereby lim

SUBMITTER: Buchman A 

PROVIDER: S-EPMC8459378 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature