Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis.

ABSTRACT: Macrophages are generally thought to play a key role in the pathogenesis of aseptic loosening through initiating periprosthetic inflammation and pathological bone resorption. The aim of this study was to identify macrophage-derived factors that promote osteoclast differentiation and periprosthetic bone destruction. To achieve this, we examined the effects of 12 macrophage-derived factors that were identified by RNA-seq analysis of stimulated macrophages on osteoclast differentiation. Surprisingly, thymidine phosphorylase (TYMP) was found to trigger significant number of osteoclasts that exhibited resorbing activities on dentine slices. Functionally, TYMP knockdown reduced the number of osteoclasts in macrophages that had been stimulated with polyethylene debris. TYMP were detected in serum

SUBMITTER: Matsumae G 

PROVIDER: S-EPMC8459589 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature