Novel Insights into Quantitative Proteomics from an Innovative Bottom-Up Simple Light Isotope Metabolic (bSLIM) Labeling Data Processing Strategy.

ABSTRACT: Simple light isotope metabolic labeling (SLIM labeling) is an innovative method to quantify variations in the proteome based on an original in vivo labeling strategy. Heterotrophic cells grown in U-[¹²C] as the sole source of carbon synthesize U-[¹²C]-amino acids, which are incorporated into proteins, giving rise to U-[¹²C]-proteins. This results in a large increase in the intensity of the monoisotope ion of peptides and proteins, thus allowing higher identification scores and protein sequence coverage in mass spectrometry experiments. This method, initially developed for signal processing and quantification of the incorporation rate of ¹²C into peptides, was based on a multistep process that was difficult to implement for many laboratorie

SUBMITTER: Senecaut N 

PROVIDER: S-EPMC8459934 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature