Looking for more reliable biomarkers in breast cancer: Comparison between routine methods and RT-qPCR.

ABSTRACT:

Purpose

Decades of quality control efforts have raised the standards of immunohistochemistry (IHC), the principle method used for biomarker testing in breast cancer; however, computational pathology and reverse transcription quantitative PCR (RT-qPCR) may also hold promise for additional substantial improvements.

Methods

Herein, we investigated discrepancies in the assessment of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and marker of proliferation Ki67 comparing routinely obtained IHC (and FISH) data (ORI) with the results of manual (REV) and semi-automated (DIA) re-evaluation of the original IHC slides and then with RNA expression data from the same tissue block using the MammaTyper® (MT) gene expression assay.

Re

SUBMITTER: Caselli E 

PROVIDER: S-EPMC8460001 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature