Identification of BiP as a CB₁ Receptor-Interacting Protein That Fine-Tunes Cannabinoid Signaling in the Mouse Brain.

ABSTRACT: Cannabinoids, the bioactive constituents of cannabis, exert a wide array of effects on the brain by engaging Type 1 cannabinoid receptor (CB₁R). Accruing evidence supports that cannabinoid action relies on context-dependent factors, such as the biological characteristics of the target cell, suggesting that cell population-intrinsic molecular cues modulate CB₁R-dependent signaling. Here, by using a yeast two-hybrid-based high-throughput screening, we identified BiP as a potential CB₁R-interacting protein. We next found that CB₁R and BiP interact specifically in vitro, and mapped the interaction site within the CB₁R C-terminal (intracellular) domain and the BiP C-terminal (substrate-binding) domain-α. BiP selectively

SUBMITTER: Costas-Insua C 

PROVIDER: S-EPMC8460140 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature