Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

ABSTRACT: Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N₂ vapour phase or in liquid nitrogen (LN) at temperatures below - 150 °C. One potential risk of cryostorage is microbial cross contamination in the LN storage tanks. The current review summarises data on the occurrence of microorganisms that may compromise the safety and quality of biological materials during long-term storage. We assess the potential for the microbial contamination of LN in storage tanks holding different biological materials based on the detection by culture-based and molecular approaches. The samples themselves,

SUBMITTER: Bajerski F 

PROVIDER: S-EPMC8460408 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature