Effectiveness of Cytochrome c Oxidase Subunit 1 Gene Nested Polymerase Chain Reaction Compared to Dermoscopy or Microscopy Alone for the Detection and Diagnosis of Sarcoptes scabiei var. hominis Infection.

ABSTRACT:

Background

Background: While microscopy (MS) evaluation of skin scrapings has a 100% positive predictive value and specificity by definition for scabies diagnosis, it has low sensitivity. Dermoscopy (DS) has not yet been widely accepted for diagnosis, and long-term clinician training is required.

Objective

To evaluate the diagnostic validity of cytochrome c oxidase subunit 1 (cox1) gene nested polymerase chain reaction (PCR) as an adjunctive method for diagnosing scabies.

Methods

This was a prospective, single institution study, conducted on a total of 302 skin lesions from 50 patients suspected of scabies at Kangdong Sacred Heart Hospital in Seoul, Korea. DS, MS, and cox1 nested PCR were performed on all patients.

Results

Of the 302 lesions, 145 (48.0%)

SUBMITTER: Yang SY 

PROVIDER: S-EPMC8460474 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature