Ozone-induced changes in the murine lung extracellular vesicle small RNA landscape.

ABSTRACT: Inhalation exposure to ozone (O₃ ) causes adverse respiratory health effects that result from airway inflammation, a complex response mediated in part by changes to airway cellular transcriptional programs. These programs may be regulated by microRNAs transferred between cells (e.g., epithelial cells and macrophages) via extracellular vesicles (EV miRNA). To explore this, we exposed female C57BL/6J mice to filtered air (FA), 1, or 2 ppm O₃ by inhalation and collected bronchoalveolar lavage fluid (BALF) 21 h later for markers of airway inflammation, EVs, and EV miRNA. Both concentrations of O₃  significantly increased markers of inflammation (neutrophils), injury (total protein), and the number of EV-sized particles in the BALF. Imagestream analysis indicate

SUBMITTER: Smith GJ 

PROVIDER: S-EPMC8461034 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature