Homologous Escherichia coli Identified in Cerebrospinal Fluid and Bloodstream.

ABSTRACT:

Background

Escherichia coli is an opportunistic bacterium that causes a wide range of diseases, such as bloodstream infection and central nervous system infection. The traditional culture-based method to detect E. coli usually takes more than 2 days. The object of this study is to explore the value of metagenomic next-generation sequencing (mNGS) in identifying E. coli from human cerebrospinal fluid. In addition, we investigated the infection source of E. coli through whole genome sequencing and phylogenetic analysis.

Methods

We combined a clinical example to analyze the function of mNGS in pathogen detection from cerebrospinal fluid. NextSeq 550Dx platform was applied for mNGS. Next, whole genome sequencing was performed to obtain the genomic cha

SUBMITTER: Shi Q 

PROVIDER: S-EPMC8461209 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature