Hsa_circ_0088212-mediated miR-520 h/APOA1 axis inhibits osteosarcoma progression.

ABSTRACT:

Background

It has been known for decades that circRNAs are deregulated in cancer. Here, we characterized the role and underlying mechanism of circ_0088212 in osteosarcoma.

Methods

The expression levels of circ_0088212, miR-520 h, and APOA1 were determined by RT-qPCR. RNase R digestion was performed to verify the circular structure of circ_0088212. CCK8 and transwell invasion assays were conducted to examine the in vitro malignancy of osteosarcoma. Caspase-3 activity was also measured. An in vivo model of osteosarcoma was constructed to examine the in vivo effect of circ_0088212 on osteosarcoma. Luciferase reporter, RNA RIP, and RNA pull-down assays were performed to verify the interaction between miR-520 h and APOA1 or circ_0088212.

Results

Circ_0088212 and APOA1 were

SUBMITTER: Liu F 

PROVIDER: S-EPMC8461379 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature