A Novel Approach for the Synthesis of Human Heteropolymer Ferritins of Different H to L Subunit Ratios.

ABSTRACT: Mammalian ferritins are predominantly heteropolymeric species consisting of 24 structurally similar, but functionally different subunit types, named H and L, that co-assemble in different proportions. Despite their discovery more than 8 decades ago, recombinant human heteropolymer ferritins have never been synthesized, owing to the lack of a good expression system. Here, we describe for the first time a unique approach that uses a novel plasmid design that enables the synthesis of these complex ferritin nanostructures. Our study reveals an original system that can be easily tuned by altering the concentrations of two inducers, allowing the synthesis of a full spectrum of heteropolymer ferritins, from H-rich to L-rich ferritins and any combinations in-between (isoferritins). The H to L subu

SUBMITTER: Srivastava AK 

PROVIDER: S-EPMC8461589 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature